RESUMO
Seven strains belonging to genus Pseudomonas were isolated from an enrichment with hydrocarbon mixtures. Tests for enzyme activities showed that five strains used predominantly the catabolic meta-pathway for aromatic hydrocarbon degradation. Furthermore, the xylE gene which encodes a catechol 2,3-dioxygenase was amplified by PCR, and in two strains the nahAc gene, a key enzyme for naphthalene catabolism, was also found. The xylE gene might be a good marker to identify aromatic hydrocarbon degrading bacteria in soils from Patagonia.
Assuntos
Dioxigenases , Genes Bacterianos , Hidrocarbonetos Aromáticos/metabolismo , Pseudomonas/genética , Microbiologia do Solo , Argentina , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Biodegradação Ambiental , Catecol 1,2-Dioxigenase , Catecol 2,3-Dioxigenase , DNA Bacteriano/genética , Indução Enzimática , Naftalenos/metabolismo , Oxigenases/genética , Oxigenases/metabolismo , Reação em Cadeia da Polimerase , Protocatecoate-3,4-Dioxigenase/genética , Protocatecoate-3,4-Dioxigenase/metabolismo , Pseudomonas/classificação , Pseudomonas/enzimologia , Pseudomonas/isolamento & purificação , Poluentes do Solo/metabolismoRESUMO
Seven strains belonging to genus Pseudomonas were isolated from an enrichment with hydrocarbon mixtures. Tests for enzyme activities showed that five strains used predominantly the catabolic meta-pathway for aromatic hydrocarbon degradation. Furthermore, the xylE gene which encodes a catechol 2,3-dioxygenase was amplified by PCR, and in two strains the nahAc gene, a key enzyme for naphthalene catabolism, was also found. The xylE gene might be a good marker to identify aromatic hydrocarbon degrading bacteria in soils from Patagonia.
Assuntos
Genes Bacterianos , Hidrocarbonetos Aromáticos/metabolismo , Pseudomonas , Microbiologia do Solo , Argentina , Biodegradação Ambiental , DNA Bacteriano , Indução Enzimática , Naftalenos , Oxigenases , Reação em Cadeia da Polimerase , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Protocatecoate-3,4-Dioxigenase , Pseudomonas , Poluentes do SoloRESUMO
Seven strains belonging to genus Pseudomonas were isolated from an enrichment with hydrocarbon mixtures. Tests for enzyme activities showed that five strains used predominantly the catabolic meta-pathway for aromatic hydrocarbon degradation. Furthermore, the xylE gene which encodes a catechol 2,3-dioxygenase was amplified by PCR, and in two strains the nahAc gene, a key enzyme for naphthalene catabolism, was also found. The xylE gene might be a good marker to identify aromatic hydrocarbon degrading bacteria in soils from Patagonia.(AU)
Assuntos
Estudo Comparativo , Genes Bacterianos , Hidrocarbonetos Aromáticos/metabolismo , Pseudomonas/genética , Microbiologia do Solo , Argentina , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , DNA Bacteriano/genética , Indução Enzimática , Naftalenos/metabolismo , Oxigenases/genética , Oxigenases/metabolismo , Reação em Cadeia da Polimerase , Protocatecoate-3,4-Dioxigenase/genética , Protocatecoate-3,4-Dioxigenase/metabolismo , Pseudomonas/classificação , Pseudomonas/enzimologia , Pseudomonas/isolamento & purificação , Poluentes do Solo/metabolismoRESUMO
Seven strains belonging to genus Pseudomonas were isolated from an enrichment with hydrocarbon mixtures. Tests for enzyme activities showed that five strains used predominantly the catabolic meta-pathway for aromatic hydrocarbon degradation. Furthermore, the xylE gene which encodes a catechol 2,3-dioxygenase was amplified by PCR, and in two strains the nahAc gene, a key enzyme for naphthalene catabolism, was also found. The xylE gene might be a good marker to identify aromatic hydrocarbon degrading bacteria in soils from Patagonia.
